Indiana University Purdue University Indianapolis

A.J. Baucum Ph.D.

Assistant Professor, Biology

Education

Ph.D., Department of Pharmacology and Toxicology, GPA 3.895, University of Utah, Salt Lake City, UT.

2004

B.S., Magna Cum Laude; Major: Biology, Minor: Spanish. Honors Program, GPA: 3.79.  Loyola Marymount University, Los Angeles, CA

1999

Research in the Baucum laboratory focuses on the identification and characterization of synaptic protein complexes in a brain region called the striatum. The striatum is critical in allowing for fine motor control as well as being responsible for multiple effects of drugs of abuse. A decrease in dopamine innervation to the striatum underlies motor deficits associated with normal aging and age-related pathological conditions such as Parkinson Disease. Moreover, enhanced dopaminergic tone via blockade of dopamine reuptake or enhanced dopamine release caused by drugs of abuse such as cocaine and amphetamine can lead to motor and euphoric effects. How modulation of dopaminergic tone effects synaptic signaling and synaptic protein complexes in striatal medium spiny neurons has not been fully characterized.

The lab focuses on a synaptic protein, spinophilin, which is known to act as a scaffold, brining multiple proteins together to allow for efficient organization and signalling. In particular, spinophilin binds to F-actin and can regulate actin and spine dynamics. Also, it binds to protein phosphatase 1 to target this phosphatase to various substrates, allowing for modulation of normal signaling. In collaboration with the proteomics core facility at IUPUI, we will be using mass spectrometry-based technologies to identify and quantify changes in specific spinophilin post-translational modifications and protein-protein interactions in animal models of Parkinson's Disease and following exposure to drugs of abuse. These studies will be followed up with biochemical and imaging-based technologies to further characterize and understand the role that altered spinophilin signaling and protein complexes have on regulating changes observed under pathological conditions.

Professional Affiliations

Member, American Society for Mass Spectrometry

2011 – present

Member, American Society for Pharmacology and Experimental Therapeutics

2007 – present

Member, Society for Neuroscience

2004 – present

Current Research

Current research in the laboratory utilizes immunoprecipitation techniques, western blotting, imaging, and proteomics-based studies to characterize spinophilin protein complexes in health and disease.

Select Publications

Brian C. Shonesy, Xiaohan Wang, Teniel S. Ramikie, Victoria S. Cavener, Tyler Rentz, Anthony J. Baucum II, Nidhi Jalan-Sakrikar, Kristie L. Rose, Danny G. Winder, Sachin Patel, Roger J. Colbran. CaMKII is a novel diacylglycerol lipase-alpha interacting protein that regulates striatal endocannabinoid signaling. Nat Neurosci 2013 Apr 16(4):456-63.

Anthony J. Baucum II, Abigail M. Brown, Roger J. Colbran. Differential association of postsynaptic signaling protein complexes in striatum and hippocampus. J. Neurochem 2013 Feb; 124(4):490-501

Anthony J. Baucum II, Stefan Strack, and Roger J. Colbran. Age-Dependent Targeting of Protein Phosphatase 1 to Ca2+/Calmodulin-Dependent Protein Kinase II by Spinophilin in Mouse Striatum. PLoS One. 2012;7(2):e31554

Anthony J. Baucum II, Nidhi Jalan-Sakrikar, Yuxia Jiao, Richard M. Gustin, Leigh C. Carmody, David L. Tabb, Amy-Joan L. Ham, Roger J. Colbran.  Identification and validation of novel spinophilin-associated proteins in rodent striatum using an enhanced ex vivo shotgun proteomics approach.  Mol Cell Proteomics. 2010 Jun;9(6):1243-59.

Anthony J. Baucum II, Kristi S. Rau, Evan L. Riddle, Glen R. Hanson, Annette E. Fleckenstein.  “Methamphetamine Increases Dopamine Transporter Complex Formation via a Dopamine- and Hyperthermia-Associated Mechanism”  J Neurosci. 2004 Mar 31;24(13):3436-3443.

Awards & Honors

Extramural

2nd place, Neuropharmacology Division of ASPET postdoctoral award competition, Experimental Biology Meeting

2009

Neuroscience Scholars Award, Society for Neuroscience

2007 – 2009

Intramural

Best poster award Vanderbilt Kennedy Center Science Day, cellular and molecular neuroscience postdoc division.

2011

Vanderbilt University Medical Center Postdoc of the Year

2010

Best poster award Vanderbilt Postdoc Poster Symposium – Neuroscience division

2010

Best poster award Vanderbilt Brain Institute Neuroscience Retreat

2009

Best poster award Vanderbilt Kennedy Center Science Day, cellular and molecular neuroscience postdoc division.

2009

Best poster award Vanderbilt Postdoc Poster Symposium – Neuroscience division

2009

GRANT FUNDING
Current

NINDS K01 – K01NS073700

2012-2017

Completed

UNCF-Merck Postdoctoral Fellowship

2007-2009

Neurogenomics Training Grant 5T32MH065215-04

2006-2007